IPTG (Dioxane-Free) 价格_品牌:Ambion-丁香通

Form:	Powder
Chemical Name:	IPTG (Isopropyl β-D-thiogalactopyranoside)
Product Size:	5 g
Shipping Condition:	Room Temperature
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

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文献和实验

相关实验

IPTG 诱导蛋白表达实验
状态.此时,I序列在PI启动序列操纵下表达的Lac阻遏蛋白与O序列结合,阻碍RNA聚合酶与P序列结合,抑制转录起动. 当有乳糖存在时,lac操纵子(元)即可被诱导.在这个操纵子(元)体系中,真正的诱导剂并非乳糖本身.乳糖进入细胞,经b-半乳糖苷酶催化,转变为半乳糖.后者作为一种诱导剂分子结合阻遏蛋白,使蛋白构象变化,导致阻遏蛋白与O序列解离、发生转录.异丙基硫代半乳糖苷(IPTG)是一种作用极强的诱导剂,不被细菌代谢而十分稳定,因此被实验室广泛应用. 材料： 1、诱导
About LacZ,IPTG and X-Gal
载体操作中最常用的几个概念： 1. The lac Z gene may be used as a simple "reporter" gene, separated from its natural promoter. For example, we might put the lac Z gene in front of a mammalian promoter
The Effects of Calcium-Free Seawater on the Development of Sea Urchin Embryos
Objective The purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were placed in calcium-free sea after fertilization and at the initiation of gastrulation

技术资料

IPTG (Dioxane-Free)