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- 文献和实验
- 技术资料
- 抗体英文名：
IC Fixation Buffer
- 规格：
125ml
cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333),
when performing intracellular staining of antigens located in the cytoplasm and
secretory pathway, such as cytokines, chemokines and growth factors. This buffer,
when used as instructed, is compatible with surface staining fluorochrome conjugated
antibodies and can be used as a short term storage buffer for
fluorochrome (including tandem dyes)-stained cells for up to 3 days at 2-8oC (stored in the dark).
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文献和实验Methanol Fixation for Immunofluorescence
. We recommend that you try both types of fixation for your particular antibody/fusion protein when working out your initial immunofluorescence conditions. MeS Buffer 100 mM MeS, pH 6.9 1 mM EGTA 1 mM MgCl2 Store at 4°C Methanol Fix (100
Pouring Linear and Buffer-Gradient Sequencing Gels
to be read from a single run) up to 100 cm in length can be poured, as can wider gels (to enable more clones to be loaded onto a single gel). However, these larger gels are more difficult to handle after electrophoresis, i.e., during fixation, drying
tightly, and warm in a water bath. 5. Cytoskeleton buffer with 0.5 mg/ml NaBH4 , 30 ml. Prepare fresh before use in a fixation box. Bubbles should be visible. Store NaBH4 desiccated in a tightly-sealed container. 6. Phosphate buffered saline
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