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- ebioscience
- 00-5333-54
- 2025年07月13日
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- 抗体英文名：
1-step Fix/Lyse Solution (10X)
- 规格：
50ml
after staining peripheral blood cells with fluorochrome conjugated antibodies.
This solution has been specially formulated to lyse non-nucleated erythrocytes
while maintaining a fixed and labeled leukocyte population. Therefore, whole
blood samples can be stained for the appropriate markers, RBC-lysed, washed,
and then analyzed by flow cytometry. Isolation of mononuclear cells by gradient
centrifugation (e.g. Ficoll-Paque density gradient separation) is not required
when using this solution. The eBioscience 1-Step Fix/Lyse Solution is compatible
with antibodies conjugated to organic dyes, eFluor® nanocrystals, and tandem dyes.
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文献和实验Multiplex PCR: Critical Parameters and Step-by-Step Protocol
. Based on this experience, a step-by-step multiplex PCR protocol was designed (Figure 1), with practical solutions to many of the problems encountered. This protocol should be useful to those using PCR technology in both the research and the clinical
Two-Step RT-PCR protocol We will provide both one-step and two-step protocols for RT -PCR . We recommend the two-step protocol for this class. In the one-step protocol, the components of RT and PCR are mixed in a single tube
Multiplex PCR: Critical Parameters and Step
. Based on this experience, a step-by-step multiplex PCR protocol was designed (Figure 1), with practical solutions to many of the problems encountered. This protocol should be useful to those using PCR technology in both the research and the clinical
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