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LAMBDA gt10 VECTOR

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  • MP
  • 04821220
  • 进口
  • 2025年07月14日
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    • 文献和实验
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    • 保存条件

      Invalid

    • 库存

      10 µg

    • 供应商

      西宝生物

    • 英文名

      LAMBDA gt10 VECTOR

    • 规格

      BIOCHEMICALS

    l gt 10 is a 43.34 Kb insertion vector with a unique EcoR I restriction site within the repressor (cl) gene. It can accept restriction fragments up to approximately 7.5 Kb in length, such as cDNA molecules with EcoR I ends. l gt 10 does not require an insert to be packageable and is thus much more efficient at cloning smaller fragments, such as cDNA molecules of 400-1500 base pairs, than other related lambda insertion vectors. l gt 10 is supplied as an aqueous solution in 10 mM Tris HCl, pH 8.0, 1 mM EDTA at a concentration of 0.5 m g/ m l.

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    相关实验
    • Construction of cDNA Libraries in gt10 or gt11

      will require about 2 � 105 clones (2 ). This can be achieved with a few micrograms of mRNA by using the efficient RNase H method of making double-stranded cDNA (3 ) and a bacterioghage λ vector that exploits the high efficiency with which in vitro packaged

    • Direct Sequencing of Inserts Cloned into Lambda Vectors

      Bacteriophage lambda is widely used as a vector for both genomic and cDNA cloning. However, although lambda is highly efficient for cloning, its use for direct sequencing is limited because of a lack of reliable sequencing methods

    • Screening lambda cDNA or genomic libraries

      ZAP II.For lambda gt11 use the host Y1090.The Barstead mixed stage C.elegans cDNA library is in the original lamda ZAP vector,and requires a host carrying the supF mutation,such as Y1090.Growing the cells:grow up 40 ml of bacteria o/n in LBspin 5 min

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