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- 技术资料
- 保存条件:
Invalid
- 库存:
10 µg
- 供应商:
西宝生物
- 英文名:
LAMBDA gt10 VECTOR
- 规格:
BIOCHEMICALS
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文献和实验Construction of cDNA Libraries in gt10 or gt11
will require about 2 � 105 clones (2 ). This can be achieved with a few micrograms of mRNA by using the efficient RNase H method of making double-stranded cDNA (3 ) and a bacterioghage λ vector that exploits the high efficiency with which in vitro packaged
Direct Sequencing of Inserts Cloned into Lambda Vectors
Bacteriophage lambda is widely used as a vector for both genomic and cDNA cloning. However, although lambda is highly efficient for cloning, its use for direct sequencing is limited because of a lack of reliable sequencing methods
Screening lambda cDNA or genomic libraries
ZAP II.For lambda gt11 use the host Y1090.The Barstead mixed stage C.elegans cDNA library is in the original lamda ZAP vector,and requires a host carrying the supF mutation,such as Y1090.Growing the cells:grow up 40 ml of bacteria o/n in LBspin 5 min
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