RNAPrep Kit - Large

Ribominus Transcriptome Isolation Kit (Yeast and Bacteria) - for efficient transcriptome enrichment by depleting large

实验原理   The large ribososmal RNA depleted RNA fraction is termed as RiboMinus™ RNA fraction. The RiboMinus™ RNA fraction contains polyadenylated (polyA) mRNA (yeast), non-polyadenylated RNA, pre-processed RNA, tRNA, smal 

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

containing chloramphenicol (Cm, 20 μg/ml)/streptomycin (Stp, 75 μg/ml) and purified using the Large construct kit (Qiagen, Valencia, CA, USA). PCR PCR reactions using oligonucleotides carrying HAs were performed using

Miniprep/Qiagen kit

  Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.