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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
. See Troubleshooting. 38. Filter the cells through a 40-µm cell strainer, and centrifuge at 400g (1500 rpm) for 5 min at 4°C. 39. Resuspend the cells in 3 mL of OP9 medium per 10-cm dish initially seeded on day
liquid chromatography, comprising a micro-column packed with three phases of chromatographic material as follows: 8.5 cm of 5 µm reversed phase material (Polaris C18-A, Metachem); 4 cm of 5 µm 300 Å strong cation exchanger (PolyLC); and lastly
线粒体荧光探针大全:TMRM,Mitotracker,JC-1
reticulum when used at higher concentrations (~5�50 µM or ~1�10 µg/mL). DiOC6 (3) (D273) stains mitochondria in live yeast 21,117�119 and other eukaryotic cells,98,120 as well as sarcoplasmic reticulum in beating heart cells.121 It has also been used
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