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文献和实验ES CELL DNA EXTRACTION: TUBE ME
off supernatant as in step 16. 19. Store the open tube on the bench at TRm until the last traces of fluid have evaporated. (i.e.: Air dry). 20. Dissolve the DNA in 30µl (or 50 µl, and use half of sample) of 10mM Tris pH 8.5 (or TE). (pipet
the pellet.Vacuum droplets from walls of tube as well. 17.Half fill the tube with 70% EtOH and re-spin for 2 minutes at 4°ree;C. 18.Suck off supernatant as in step 16. 19.Store the open tube on the bench at TRm until the last traces of fluid
ES CELL DNA EXTRACTION: TUBE ME
, with vacuum flask). Do not disturb the pellet. Vacuum droplets from walls of tube as well. 17. Half fill the tube with 70% EtOH and re-spin for 2 minutes at 4℃. 18. Suck off supernatant as in step 16. 19. Store the open tube on the bench at TRm
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