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文献和实验GENERAL MAINTENANCE INSTRUCTIONS FOR IMAGINAL DISC CELL LINES
can be frozen in complete M3 medium + 10% FCS and 10% DMSO; again use subconfluent cultures. MAKING FLY EXTRACT FOR WING DISC CELL MAINTENANCE (Cullen and Milner, TISSUE AND CELL, 1991 23(1):29-39.) Start with a collection of about 30g of healthy flies
RUVKUN Antibody Staining Protocol revised 10/29/90
RUVKUN Antibody Staining Protocol revised 10/29/90 Fixation: 1. C. elegans N2 grown in liquid or plates. Harvest worms. Wash for 2 hours at room temp. in 1X M9 or PBS. 2. Fix in 5 ml of FRESH THAT DAY 1% paraformaldehyde:
, at 20,000 RPM (k=402), the time required to sediment a particle of100 S, in water at 20 ° C is calculated by: t = 402/100or 4.02 hours (4 hrs, 1 min) Table F.2 presents the k values for the Sorvall SS-34 rotor and for the Sorvall GSA rotor
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