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- 2026年07月17日
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文献和实验because it is often the buffer used during the first aldehyde fixation. 5) Post fix in 2% osmium tetroxide in 100 mM cacodylate buffer (1 hr on ice). Take care with the osmium tetroxide, even the fumes are dangerous. 6). Wash in water (3 x 5'). 7). Wash in 50 mM
Immunofluorescence on ultrathin resin sections
. Residual water is drained with filter paper along the outside of the loop. Note: air-dried resin sections can be stored for months prior to labelling. Procedure: Encircle sections
Coupling Peptides To Resin For Affinity Purification
is light sensitive. Wash with 10 volumes of 0.1 M NaPi pH 7.8= buffer. Resuspend the resin as a 50% slurry in buffer. Add the peptide. If the peptide is soluble directly in buffer, add it as a solid. Many peptides go in better added as a 100
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