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文献和实验All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
Brief protocol using Digest-All™ Kit is described below. The Digest-All™ is a flexible and standardized system for tissue digestion and contains trypsin, ficin, and pepsin proteolytic enzymes. The degree of digestion is based on a standardized 10 minute
(3 ), “megaprimer PCR”(4 –7 ), marker-coupled PCR (8 ), and so on, introducing changes to DNA sequences has become less tedious and more efficient. Recently, marketed site-directed mutagenesis (SDM) kits, such as Transformer™ Site-Directed Mutagenesis
【转贴】immunofluorescence staining
Kit (Molecular Probes), coverslips can (optionally) be incubated with 1-2 drops of Equilibration Buffer for 5-10 min. before mounting.Place a drop of mounting media on the slide for each coverslip to be mounted (two drops for square coverslips
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