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文献和实验Synthesis of Peptide‐Oligonucleotide Conjugates by Diels‐Alder Cycloaddition in Water
RNase‐free water: mix Milli‐Q water vigorously with 0.1% DEPC overnight and autoclave twice, or obtain RNase‐free water from Milli‐Q system equipped with a 5000‐Da ultrafiltration cartridge
Nucleobond Column BAC DNA Purification for Transgenic Mouse Production
. Put the folded filter onto a 50 ml tube or equilibrated Nucleobond AX cartridge, wet it with 0.5 - 1.0 ml of water and fill it with the cooled lysate. Collect the cleared flowthrough. These filters are suitable for plasmid isolation with Nucleobond AX 100
Microinjection DNA Purification
Centigrade for 10 minutes, vigorously vortexing every 2 to 3 minutes. 7. Place a NucleoSpinTM cartridge in a 2 ml micro tube and load 750 microliters dissolved gel slice onto the cartridge. Spin at maximum speed for 60 seconds in a microcentrifuge
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