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文献和实验96-well Plate Dual Luciferase Assay--96孔板荧光素酶检测
Reagents: Plasmids: can be found in my plasmid(1) box in the ?20oC freezer. 985 : FR-tk-luciferase 2517 : Renilla-tk-luciferase 2145 : GFP Lipfectamine and lipofectamine plus reagents: in the upper shelf of the medium freezer in tissue
Preparing Overnight Bacterial Culture
overnight at 37 degrees C Supplies: Shaker at 37 degrees C Procedures: Scratch a single colony from the agar plate with bacterial colonies using a sterile small micropipetter tip (use a flamed forceps to hold the tip
on top of the larger plate and spacers.Secure the plates with a casting boot (tape or clamps may be substituted for the casting boot).C: Polymerize the Gel TIP: Acrylamide is a neurotoxin. Be sure to wear gloves and a labcoat when working with this substance. Add 480
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