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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
as you finish washing them. Note : Always ensure that plates are neatly vertically stacked and aligned. The weight of a stack of poured plates (200-250 grams/plate) is considerable, and any misalignment when they are stacked will eventually lead to cracked
96-well RNA In Situ Hybridization Protocol
vendor information is described in section IV.1. Place 5μlof linearized plasmid (100 - 500ng) into 96 well plate. (The CK cDNA miniprep DNA was linearized with Apa I). If restriction buffer is compatible with the polymerase buffer, 5μlof restriction
cells (hESC) are difficult to adapt to 96?well plate assays, such as the MTT assay, because they survive best when plated as colonies, which are not easily counted and plated accurately. Two methods were developed to address this problem
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