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文献和实验Preparing Overnight Bacterial Culture
overnight at 37 degrees C Supplies: Shaker at 37 degrees C Procedures: Scratch a single colony from the agar plate with bacterial colonies using a sterile small micropipetter tip (use a flamed forceps to hold the tip
on top of the larger plate and spacers.Secure the plates with a casting boot (tape or clamps may be substituted for the casting boot).C: Polymerize the Gel TIP: Acrylamide is a neurotoxin. Be sure to wear gloves and a labcoat when working with this substance. Add 480
Gene Knockout Techniques(1) The Knockout Process
with its own pipette tip (one box of tips per digestion plate).The tricks:Add the harvest solution using a repeat pipetter, 12 wells (one plate) at a time.To get good representation in each population sample, swirl the plate gently after adding the harvest solution
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