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文献和实验Pulsed EPR Distance Measurements in Soluble Proteins by Site‐Directed Spin Labeling (SDSL)
of pulsed electron paramagnetic resonance (EPR) in structural biology centers on recent improvements in distance measurements using the double electron?electron resonance (DEER) technique. This unit focuses on EPR?based distance measurements by site?directed
))where T = time in minutesD = radial distance in cm for rL = radial distance to meniscusN = viscosity of the fluid mediumg = density of the fluid mediump = density of the particle to sedimentd = diameter of the particle in cms.S = rotational velocity
of a given particle, then the time needed to completely sediement a particle can be determined by the formula: T = ((D-L)/(D+L))*(N/(d (g-p)S )) where T = time in minutes D = radial distance in cm for r L = radial distance to meniscus N
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