- 询价
- merck-millipore
- MRCF0R100
- 2025年07月10日
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文献和实验Fast and reliable mini-prep RNA extraction from Neurospora crassa
otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml
An Ultra-fast method of DNA extraction from Neurospora
diatomaceous earth (Sigma D-5384), added to 100 ml of 6M Guanidine Thiocyanate (ultrapure, BMB) Stable for approximately 6 months at room temperature. The yield of DNA varies from 5-30 µg depending upon the amount of mycelium that is harvested. The quality
E.Z.N.A.TM DNA Fast Protocol For FFPE Tissue
Protease. 4. Add 20 ul OB Protease and mix by vortexing. Then Incubate at 55°C for 1-3 hours or overnight. 5. Briefly centrifuge the tube to collect any drops from the inside of the lid. If RNAFree genomic DNA if required, add 2 ul Rnase(100mg
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