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文献和实验E.Z.N.A.TM DNA Fast Protocol For FFPE Tissue
2 ml collection tube and wash by pipetting 500 ul of Buffer HB. Centrifuge at 10,000 x g for 1 min. Again, dispose of collection tube and flow-through liquid. 10. Place the column into a new 2 ml collection tube and wash by pipetting 500 ul of DNA
have been used to obtain enriched or purified cell populations, including flow cell sorting. Current protocols are usually time-consuming and may imply loss of short-lived RNAs, which is undesirable for expression profiling. We describe an optimized method
1.1 ). with and without 100 µg/ml carbenicillin (Sigma, cat. no C1389) TALEN Expression Vectors (available through Addgene: http://www.addgene.org/talengineering
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