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文献和实验E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
as above. This step ensures that residual protein contamination is removed and must be included for downstream application requiring high quality DNA. 12. Discard the flow-through liquid and wash the column by adding 700 u DNA Wash Buffer diluted with ethanol
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
through the column. 11. Wash column with 500 ul Buffer HB and centrifuge as above. This step ensures that residual protein contamination is removed and must be included for downstream application requiring high quality DNA. 12. Discard the flow
technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical
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