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上海恪敏生物科技有限公司
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文献和实验问: 1.DEAE FAST FLOW经过不同盐浓度洗脱后,在用初始缓冲液平衡柱子过程中,缓冲液的体积和平衡时间与什么有关?我要的蛋白等电点是4.5,用的是PH7.5的20mMTris-HCl缓冲液平衡,而且我用的是5ML的预装柱,洗脱后平衡得需要大约120ML的缓冲液,我看说明书写的只需5-6个柱体积,这是为什么呢? 2.如果自己装的DEAE FAST
have been used to obtain enriched or purified cell populations, including flow cell sorting. Current protocols are usually time-consuming and may imply loss of short-lived RNAs, which is undesirable for expression profiling. We describe an optimized method
In Vivo Detection of Neurotransmitters with Fast Cyclic Voltammetry
Electrochemical techniques detect materials by oxidizing them in solution at a positively polarized “working” electrode. The oxidized material gives up electrons that are collected by the working electrode and generate a current flow
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