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- 2025年09月08日
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- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
Seebio
- 英文名：
Cell Extraction buffer
- 规格：
100 mL
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文献和实验CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
ES CELL DNA EXTRACTION: TUBE ME
in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM Tris-HCl pH7.5 - 0.5 ml of 2M 10 mM EDTA - 2 ml of 0.5M 10 mM NaCl - 0.2 ml
Simplified DNA Extraction from Cell or Tissue
Purpose DNA extraction without phenol extraction and centrifugation. Procedure 1. Lysis: the lysis buffer (usually 0.5ml) is added to the tissue or cell ( for a 75cm2 flask, add 5ml directly to the cell). Digestion is complete
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