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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
seebio
- 英文名:
Cell cycle staining buffer
- 保存条件:
2147483647
- 库存:
大量
- 规格:
50ml/50T
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文献和实验Cell Cycle Staining Protocol-DAPI
. 4. Fix in this final 70% EtoH solution for at least 30 min. The cells can remain in this solution for up to one week. 5. Dilute EtOH/cell suspension with 12mL PBS (for a total volume of 15mL). Spin at 2000-2200 rpm for 10 min spin. Cells
FIXATION and DNA Staining for Cell Cycle Analysis
-helix. Ribonuclease-A is used to eliminate the staining of double-stranded RNA.Generally, the method can be combined with the procedures for direct labeling or indirect labeling of cell surface antigens. Use only FITC-labeled antibodies since PI emits
A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
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