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文献和实验Following PCR, you often want to get rid of the PCR primers and Taq polymerase before the next step. This is necessary for sequencing PCR products or TA cloning. There is no clean up needed following genotyping reactions. PCR reactions
a mixture of specific and nonspecific products, the quick and clean (QC) cloning procedure was developed to clone specific products only. QC cloning is a ligation-independent cloning procedure that relies on the exonuclease activity of T4 DNA polymerase
for downstream digests oheat 5 minutes at 60 degrees ospin 2 min @ full speed in microcentrifuge oremove DNA-containing supernatant to clean tube Notes & Misc: •Conveinient PCR clean up before digestion: 100μl PCR reaction cleaned up with 50μl silica
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