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文献和实验Preparing a Selenomethionyl Protein
Purpose The protocol describes how to prepare selenomethionyl (Se-Met) protein using a regular E.coli strain. Selenium can be used for phase determination in multi-wavelength anomalous diffraction (MAD) method. Se-Met can often replace
Protein Purification HD exon 1, 16, 38, 56, and 82 CAGs fused with GST. Making constructs. Exon I of HD gene was amplified from partial cDNA constructs obtained from normal (16 CAGs) and patient (48 CAGs). The primers
GST Fusion Protein Preparation
: 0.25g/10ml,use 1:1000 only for cells with [pREP-4] plasmid!ORDERING INFOSigma: Glutathione beads,cat # G-4510Sigma: Thrombin,3560 NIH units/mg protein,cat # T-6759ICN: AEBSF: #193503NOTESbME should be freshly added when called for,as it is rapidly
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