| Human Apoptosis miRNA PCR Array: MIHS-114Z |
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The Human Apoptosis miScript miRNA PCR Array profiles the expression of 84 miRNAs that regulate programmed cell death by inhibiting pro-apoptotic and anti-apoptotic gene expression. This array provides researchers with a convenient way to quickly analyze the miRNAs most relevant to apoptosis. Apoptosis plays a critical role in normal biological processes requiring cell removal including differentiation, development, and homeostasis. Stress responses (such as heat shock, ischemia, unfolded proteins, and viral infection) cause badly damaged cells to undergo apoptosis. In cell culture, growth factor withdrawal and many known experimental compounds have a similar effect. An acquired defect in apoptosis activation often leads to uncontrolled cell growth, oncogenesis, and cancer. Ligand-bound tumor necrosis factor (TNF) receptors initiate apoptosis by recruiting FADD and other death domain adaptor proteins that then recruit and activate caspases. Environmental stresses trigger BCL2 protein oligomerization and insertion into the mitochondrial membrane, releasing APAF1 and other CARD family members that also oligomerize to recruit and activate caspases. Caspases promote a proteolytic cascade that degrades cellular protein targets, while the IAP protein family directly inhibits caspases. Besides these post-translational events, miRNAs provide another mechanism of regulating apoptosis by attenuating gene expression. This array represents miRNAs experimentally verified to regulate many pro- and anti-apoptotic genes and to stimulate or attenuate apoptosis upon their overexpression or inhibiton. Monitoring their expression helps determine the mechanisms behind programmed cell death in any model system and the propensity of a cell type to undergo apoptosis normally. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR® Green real-time PCR, the expression of a focused panel of miRNAs related to apoptosis can be easily and reliably analyzed with this miScript miRNA PCR Array.
miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available. |
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Anti-Apoptotic: miR-106b-5p, miR-141-3p, miR-183-5p, miR-186-3p, miR-210, miR-214-3p, miR-338-3p, miR-378a-3p, miR-98-5p, miR-133a, miR-181a-5p, miR-21-5p, miR-25-3p, miR-30a-5p, miR-30b-5p, miR-30d-5p.
Pro-Apoptotic: miR-128, miR-143-3p, miR-144-3p, miR-200c-3p, miR-205-5p, miR-206, miR-212-3p, miR-218-5p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-30c-5p, miR-30e-5p, miR-31-5p, miR-365a-3p, miR-409-3p, miR-449a, miR-708-5p, let-7c, let-7g, miR-1, miR-101-3p, miR-133b, miR-149-3p, miR-153, miR-15b-5p, miR-16-5p, miR-181b-5p, miR-195-5p, miR-203a, miR-204-5p, miR-29a-3p, miR-29c-3p, miR-34a-5p, miR-497-5p, miR-512-5p.
Either Anti-Apoptotic or Pro-Apoptotic: let-7e-5p, miR-23a-3p, miR-24-3p, miR-34c-5p, let-7a-5p, miR-29b-3p.
Targets Anti-Apoptotic Genes: let-7c, let-7g, miR-1, miR-101-3p, miR-122-5p, miR-133a, miR-133b, miR-193b, miR-194-5p, miR-204-5p, miR-491-5p, miR-512-5p, miR-542-3p, miR-7-5p, miR-9-5p.
Targets Pro-Apoptotic Genes: miR-134, miR-221-3p, miR-222-3p, miR-32-5p.
Targets Both Anti-Apoptotic & Pro-Apoptotic Genes: let-7a-5p, miR-125a-5p, miR-125b-5p, miR-1285-3p, miR-145-5p, miR-146a-5p, miR-149-3p, miR-153, miR-15a-5p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-181a-5p, miR-181b-5p, miR-181c-5p, miR-181d, miR-185-5p, miR-192-5p, miR-193a-5p, miR-195-5p, miR-203a, miR-20a-5p, miR-21-5p, miR-25-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30d-5p, miR-34a-5p, miR-451a, miR-497-5p, miR-92a-3p
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