细胞凋亡miRNA PCR芯片 Apoptosis miSc

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Apoptosis miScript miRNA PCR Array细胞凋亡miRNA PCR芯片

Apoptosis miScript miRNA PCR Array细胞凋亡miRNA PCR芯片

Product	Species	Technology	Cat. No.
Apoptosis miScript miRNA PCR Array	Human	miRNA Expression	MIHS-114Z
Apoptosis miScript miRNA PCR Array	Mouse	miRNA Expression	MIMM-114Z
Apoptosis miScript miRNA PCR Array	Rat	miRNA Expression	MIRN-114Z

The Apoptosis miScript miRNA PCR Array profiles the expression of 84 miRNAs that regulate programmed cell death by inhibiting pro-apoptotic and anti-apoptotic gene expression. This array provides researchers with a convenient way to quickly analyze the miRNAs most relevant to apoptosis. Apoptosis plays a critical role in normal biological processes requiring cell removal including differentiation, development, and homeostasis. Stress responses (such as heat shock, ischemia, unfolded proteins, and viral infection) cause badly damaged cells to undergo apoptosis. In cell culture, growth factor withdrawal and many known experimental compounds have a similar effect. An acquired defect in apoptosis activation often leads to uncontrolled cell growth, oncogenesis, and cancer. Ligand-bound tumor necrosis factor (TNF) receptors initiate apoptosis by recruiting FADD and other death domain adaptor proteins that then recruit and activate caspases. Environmental stresses trigger BCL2 protein oligomerization and insertion into the mitochondrial membrane, releasing APAF1 and other CARD family members that also oligomerize to recruit and activate caspases. Caspases promote a proteolytic cascade that degrades cellular protein targets, while the IAP protein family directly inhibits caspases. Besides these post-translational events, miRNAs provide another mechanism of regulating apoptosis by attenuating gene expression. This array represents miRNAs experimentally verified to regulate many pro- and anti-apoptotic genes and to stimulate or attenuate apoptosis upon their overexpression or inhibiton. Monitoring their expression helps determine the mechanisms behind programmed cell death in any model system and the propensity of a cell type to undergo apoptosis normally. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR® Green real-time PCR, the expression of a focused panel of miRNAs related to apoptosis can be easily and reliably analyzed with this miScript miRNA PCR Array.
miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease
细胞凋亡miRNA PCR芯片检测84个通过抑制凋亡激活和抗凋亡基因来调节细胞程序性死亡的microRNA的表达。这个芯片为研究人员提供了一个方便的方法来快速分析与细胞凋亡相关的microRNA。凋亡细胞在正常生物过程中起着至关重要的作用，包括分化、发育和内稳态的调节。应激反应(如热休克、缺血,展开的蛋白质,和病毒感染)引起严重受损的细胞发生凋亡。在细胞培养中生长因子消退和许多已知的实验化合物也有相似的效果。后天的细胞凋亡激活缺陷往往导致不可控的细胞生长,肿瘤形成,和癌症。配体肿瘤坏死因子(TNF)受体启动细胞凋亡通过招募FADD和其他死亡结构域接头蛋白,然后招募和激活凋亡蛋白酶。环境压力引发BCL2蛋白质的寡聚化和插入到线粒体膜,释放APAF1和其他寡聚化的CARD家族成员，来招募和激活凋亡蛋白酶。凋亡蛋白酶促进蛋白质级联水解,来降解细胞蛋白质,而IAP蛋白质家族直接抑制凋亡蛋白酶。除了这些翻译后调控,microRNA提供另一种衰减基因表达来调节细胞凋亡的机制。这个芯片的microRNA在实验上已证实，来调控许多促进和抑制凋亡的基因，通过过表达或抑制来促进或减弱凋亡。检测他们的表达有助于确定细胞程序性死亡背后的机制在任何模型系统和经细胞凋亡过程的细胞类型倾向。每个芯片上有一组对照组，其数据分析可使用ΔΔCT相对量化的方法,评估逆转录性能和聚合酶链反应性能的评估。使用SYBR®GREEN实时PCR和microrna qPCR芯片,可以很容易地、可靠地分析与细胞凋亡有关的miRNA。
miScript microrna的PCR芯片用于分子生物学的应用程序。本产品不用于诊断,预防和治疗的疾病。
Anti-Apoptotic:miR-106b-5p, miR-141-3p, miR-183-5p, miR-186-3p, miR-210, miR-214-3p, miR-338-3p, miR-378a-3p, miR-98-5p, miR-133a, miR-181a-5p, miR-21-5p, miR-25-3p, miR-30a-5p, miR-30b-5p, miR-30d-5p.
Pro-Apoptotic:miR-128, miR-143-3p, miR-144-3p, miR-200c-3p, miR-205-5p, miR-206, miR-212-3p, miR-218-5p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-30c-5p, miR-30e-5p, miR-31-5p, miR-365a-3p, miR-409-3p, miR-449a, miR-708-5p, let-7c, let-7g, miR-1, miR-101-3p, miR-133b, miR-149-3p, miR-153, miR-15b-5p, miR-16-5p, miR-181b-5p, miR-195-5p, miR-203a, miR-204-5p, miR-29a-3p, miR-29c-3p, miR-34a-5p, miR-497-5p, miR-512-5p.
Either Anti-Apoptotic or Pro-Apoptotic:let-7e-5p, miR-23a-3p, miR-24-3p, miR-34c-5p, let-7a-5p, miR-29b-3p.
Targets Anti-Apoptotic Genes:let-7c, let-7g, miR-1, miR-101-3p, miR-122-5p, miR-133a, miR-133b, miR-193b, miR-194-5p, miR-204-5p, miR-491-5p, miR-512-5p, miR-542-3p, miR-7-5p, miR-9-5p.
Targets Pro-Apoptotic Genes:miR-134, miR-221-3p, miR-222-3p, miR-32-5p.
Targets Both Anti-Apoptotic & Pro-Apoptotic Genes:let-7a-5p, miR-125a-5p, miR-125b-5p, miR-1285-3p, miR-145-5p, miR-146a-5p, miR-149-3p, miR-153, miR-15a-5p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-181a-5p, miR-181b-5p, miR-181c-5p, miR-181d, miR-185-5p, miR-192-5p, miR-193a-5p, miR-195-5p, miR-203a, miR-20a-5p, miR-21-5p, miR-25-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30d-5p, miR-34a-5p, miR-451a, miR-497-5p, miR-92a-3p

实验原理与流程

What miScript miRNA PCR Arrays offer?
Guaranteed Performance* - ready-to-use for gene expression analysis
Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template