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乳腺癌甲基化芯片Breast Cancer DNA Meth

ylation PCR Array
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  • Breast Cancer DNA Methylation PCR Array
  • 上海
  • 2026年01月09日
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      Breast Cancer DNA Methylation PCR Array

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      SAB

    Human Breast Cancer DNA Methylation PCR Array, Signature Panel: EAHS-011Z
    The Human Breast Cancer EpiTect Methyl II Signature PCR Array profiles the methylation status of 22 tumor suppressor gene promoters whose hypermethylation has been reported in the literature to occur frequently in a variety of breast tumors. Profiling tumor samples with these arrays may help correlate CpG island methylation status with biological phenotypes. The results may also provide insights into the molecular mechanisms and biological pathways behind oncogenesis and cancer pathology. With a simple restriction enzyme digestion and real-time PCR, research studies can analyze the methylation status of 22 different breast cancer genes with this DNA methylation PCR array.
    Both 96-well and 384-well ( 4 X 96 ) formats are available.
    The EpiTect Methyl II PCR Arrays use the MethylScreen™ Technology provided under license from Orion Genomics, LLC.
    Cell Cycle, Growth, Differentiation & Development: BRCA1, CCNA1, CCND2, CDKN1C, CDKN2A, SFN (14-3-3 sigma), TP73.
    Cell Adhesion: CDH1, CDH13.
    Transcription Factors: CDKN2A, ESR1, HIC1, PRDM2, RASSF1, TP73.
    Hormone Receptors: ESR1.
    Drug Metabolism: GSTP1.
    Apoptosis & Anti-Apoptosis: CDKN2A, PYCARD, TNFRSF10C, TP73.
    DNA Methylation: MGMT, PRDM2.
    Phosphatases: PTEN.
    Prostaglandin-Endoperoxide Synthase: PTGS2.
    Extracellular Matrix Molecules: ADAM23, SLIT2, THBS1.
    Proteases & Protease Inhibitors: ADAM23, THBS1.
    Ras Associated Protein Superfamily: RASSF1.

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    相关实验
    • DNA甲基化研究方法的回顾与评价(下)

      要设立对照。该法可用于多样本、多位点甲基化的检测,样本需要量少,适于临床样本,但存在假阳性问题。 2.2.11 甲基化敏感性斑点分析(methylation sensitive dot blotassay,MS-DBA) G Clément等2005年[43]报道了一种新的方法,能够定量或半定量分析样本中的甲基化水平。这个方法的过程是:先用重亚硫酸盐处理待测DNA片段,随后以非CG区的引物行PCR扩增,将扩增产物变性后转移到尼龙膜上,用3'端DIG标记的含有2个CG(或TG)的双核苷酸探针与DNA

    • DNA Methylation Analysis in Human Cancer

      Many tumor suppressor genes (such as p16, Rb, VHL, E-cadherin , and hMLH1 ) that are silenced by mutation are also inactivated by gene silencing through DNA methylation. Characterization of genes hypermethylated in human cancers but not in normal

    • DNA Methylation Changes in Prostate Cancer

      Epigenetic alterations contribute significantly to the development and progression of prostate cancer, the most prevalent malignant tumor in males of Western industrialized countries. Here, we review recent research on DNA methylation

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