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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
12 months from date of receipt.
- 英文名:
GTPαS
- 库存:
99
- 供应商:
Biorbyt
- CAS号:
81570-51-6
- 规格:
5 x 25 ul
描述: GTPαS
别名: Guanosine-5'-(α-thio)-triphosphate, Sodium salt; (Mixture of Rp and Sp isomers)
浓度: 100 mM-110 mM
形态: solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
应用说明: Stability against decapping scavenger pyrophosphatase; Inhibition of guanylate cyclase. Spectroscopic Propertie: λmax 252 nm, ε 13.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5). Specific Ligands: Translational factor eIF4E, Bacterial diguanylate cyclase.
分子式: C10H16N5O13P3S
分子量: Theoretical MW: 539.24 g/mol (free acid); Detected MW: 538.97 g/mol (free acid)
纯度: ≥ 95% (HPLC)
SMILES: Nc1[nH]c(=O)c2c(n([C@H]3[C@@H]([C@@H]([C@H](O3)COP(=S)(O)OP(=O)(O)OP(=O)(O)O)O)O)cn2)n1
保存条件: store at -20 °C
备注: For research use only.
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文献和实验preparations, GTP can be substituted by 35 S-labeled guanosine 5′-O -(3-thio)triphosphate ([35 S]GTγS) and on agonist stimulation a stable [35 S]GTPγS-Gα complex will form and accumulate. Separation of 35 S-bound GTPγS-Gα complexes from free [35 S]GTPγS allows
[35S]GTPS Binding as an Index of Total G-Protein and G-Subtype-Specific Activation by GPCRs
preparations, GTP can be substituted by 35 S-labeled guanosine� 5′-O -(3-thio)triphosphate ([35 S]GTPγS) and on agonist stimulation a quasi-stable [35 S]GTPγS–Gα �complex forms and accumulates. Separation of [35 S]GTPγS–Gα complexes from free [35 S]GTPγS allows
, receptor protem; E, effector molecule; α, α-subumt of G protein; βγ, βγ-subunits of G protein; S, substrate, P, product.
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