- ￥2808
- Biorbyt
- 英国
- orb1942514
- 2026年08月07日
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- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
12 months from date of receipt.
- 英文名：
GTPγS tetralithium
- 库存：
99
- 供应商：
Biorbyt
- CAS号：
94825-44-2
- 规格：
1 mg
描述: GTPγS tetralithium is a non-hydrolyzable GTP analog that activates G-proteins and stabilizes proteins against degradation. It is widely used in vitro and ex vivo to study GPCR signaling, including receptor activation and desensitization assays, for both recombinant and endogenous receptor systems.
别名: Guanosine 5'-[γ-thio]triphosphate tetralithium
靶点: transporter,Others
分子式: C10H12Li4N5O13P3S
分子量: 562.98
纯度: 75.00% (May vary between batches)
SMILES: O[C@H]1[C@@H](O[C@@H]([C@H]1O)COP(OP(OP([O-])([S-])=O)([O-])=O)([O-])=O)N2C3=C(C(N=C(N3)N)=O)N=C2.[Li+].[Li+].[Li+].[Li+]
溶解度: H2O:80 mg/mL (142.1 mM)
研究领域: Metabolism Research
保存条件: -20°C
备注: For research use only.
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文献和实验GTPγS-induced Inhibition of Binding
GTPγS-induced Inhibition of Binding 1. Chemicals and Equipment DB Buffer, PB, cAMP, cAMP [2, 8- 3H] (25 Ci/mmol, 1.0 mCi/ml, 32, 000 nM), 10 mM GTPγS, Wacker silicone AR-20, Reagiergefab test tubes (Sarstedt), millipore
preparations, GTP can be substituted by 35 S-labeled guanosine 5′-O -(3-thio)triphosphate ([35 S]GTγS) and on agonist stimulation a stable [35 S]GTPγS-Gα complex will form and accumulate. Separation of 35 S-bound GTPγS-Gα complexes from free [35 S]GTPγS allows
with it. Binding assays that assess the effect of an unlabeled receptor ligand on the binding of the radiolabeled guanosine triphosphate (GTP) analog [35 S]GTPγS provide such information and may be the most sensitive assays available for determining the relative
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