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- 2025年12月30日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
Custom Genome Editing Cell Lines
- 提供商：
北京安必奇生物科技有限公司
Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. We are pleased to announce that Creative Biogene is providing new services for making engineered cell lines.
Creative Biogene can use CRISPR/Cas9 and TALEN System to make various forms of gene modification. We have years of experience in generating the following cell lines.
• Knockout cell lines
• Conditional Knockout cell lines
• Site-specific knock-in cell lines
• Gene Mutation cell lines
• Double gene knockout cell lines
• Gene tagging cell lines
Our services include:
• Construct generation
Targeting vector construction and confirmation, and select the most efficient engineered nuclease
• Stable cell clones selection
Perform transfection on target cells including HEK293 cells, CHO cells, CHO D44 cells, HeLa cells,
other tumor cells, Mouse ES cells, Human ES cells, Primary cells etc. Select cell by MACS, FACS and screen
gene-edited cell clones. Expand and cryopreserve the engineered cells.
• Gene editing evaluation
Evaluate the target stable cell clones via one or several assays such as Western Blot, ELISA, real-time PCR,
or reporter assays etc.
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文献和实验全基因组的比较基因组杂交技术介绍（Whole-Genome and Custom Fine-Tiling Array CGH）
Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome and your sample genome. NimbleGen offers two high-definition array CGH products: whole-genome array
Zinc-Finger Nucleases-Based Genome Engineering to Generate Isogenic Human Cell Lines
integration of a transgene or reporter cassette into a “neutral site” of the human genome, such as the AAVS1 locus, permits the generation of isogenic human cell lines with two major advantages over standard genetic manipulation techniques: minimal integration
Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
. View Image Figure 2. Gene editing in one‐cell embryos. Fertilized oocytes are collected from wild‐type mice and microinjected
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