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文献和实验Gel-elongation assay for type II fatty acid synthesis
1 Determination of target selectivity E. coli fatty acid synthesis (FASII) gel-elongation assay used 4 µM of [2-14 C]malonyl-CoA (60 mCi/mmol) and 20 µM of acetyl-CoA as substrates. The reaction was carried out at 37°C for 30
Gel-elongation assay for type II fatty acid s
-elongation, FASII Figure 1 Determination of target selectivity E. coli fatty acid synthesis (FASII) gel-elongation assay used 4 µM of [2-14 C]malonyl-CoA (60 mCi/mmol) and 20 µM
Exposing gels and plates containing radioactive samples to X-ray film
below which nothing can be detected. Flashing reduces this threshold. Detection of 3H. 3H cannot be detected by autoradiography unless you have 1 mCi of it in your band. Because this is almost unheard of, it is essential to impregnate a gel containing 3H
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