Recombinant Human TROP-2(Mamma

Recombinant Human TROP-2(Mammalian, 248AA, C-6His)推荐
Recombinant Human TROP-2 (248AA, C-6His) (C07M)

产品说明(Description)

Recombinant Human Tumor-associated Calcium Signal Transducer 2 is produced by our Mammalian expression system and the target gene encoding His27-Thr274 is expressed with a 6His tag at the C-terminus.
Accession #: P09758
Known as: Tumor-associated calcium signal transducer 2;Membrane component chromosome 1 surface marker 1;Cell surface glycoprotein Trop-2; TACSTD2;TROP2


制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.


质量控制(Quality Control)

Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity-Elisa:		Bioactivity-BLI:
Immobilized Human TROP-2-His(Cat#C07M) at 2μg/ml (100 μl/well) can bind Anti-Human TROP-2 mAb. The ED50 of Anti-Human TROP-2 mAb is 13.03 ng/ml.		Loaded Anti-Human TROP-2 mAb-Fc(Cat#NC045) on Protein A Biosensor, can bind Human TROP-2-His(Cat#C07M) with an affinity constant of 0.21 nM as determined in BLI assay.

复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景(Background)

Tumor associated calcium signal transducer 2 (TACSTD2, TROP-2) is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. Human and mouse TROP-2 share 87% amino acid (aa) similarity. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

电泳(SDS-PAGE)

FOR RESEARCH USE ONLY仅限科学研究