- ¥12000
- biorad
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- 1725125
- 2025年12月16日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Universal SYBR® Green Supermix
- 库存：
10
- 供应商：
研卉生物
- 规格：
5,000 x 20 µl rxns, 50 ml
iTaq™ Universal SYBR® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml) #1725125
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyesUse iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR).
- Ready-to-use mix optimized for dye-based qPCR on any real-time PCR instrument
- Contains both ROX and fluorescein dyes for universal real-time instrument compatibility (ROX-independent and ROX-dependent systems)
- Amplify genomic DNA and difficult amplicons
- Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
Instrument Compatibility
iTaq Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
| Systems | |
| Applied Biosystems |
|
| Stratagene |
|
| Eppendorf |
|
| QIAGEN |
|
| Roche |
|
| BioFire |
|
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文献和实验Vybrant® DyeCycle Green and Orange Stains
Stability Vybrant® DyeCycle™ Green stain Vybrant® DyeCycle™ Orange stain 400 μL 5 mM solution in dimethyl sulfoxide (DMSO
SYBR Green Quantitative PCR Protocol
12μl of master mix for each well, plus some excess1 Rxn: 10μl SYBR Green Mix 52 Rxn: 520μl SYBR 0.4μl Forward Primer (10μM stock) 20.8μl F
REAL TIME PCR WITH SYBR GREEN I 建议PROTOCOL
1、反应体系 25ulCocktail 20 ul: Primer FP 1 ul + Primer RP 1u + 2 * SYBR GREEN I MIX 12.5 + H2O 5.5 ul cDNA 5 ul2、Primer 浓度，需要摸索，从200nM到700nm等，设置不同浓度。3、每个引物浓度，从well 1到12设置4个样本，阳性cDNA 1和2，NTC以及H2O，做triplicate。4、若用ABI7000或7700，所有的PCR条件可设置成一致的，减少麻烦，当然，引物
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