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- 2026年01月26日
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100 µg
蛋白名称：NCKIPSD蛋白, NCKIPSD protein
蛋白构建：A DNA sequence encoding the mature form of human NCKIPSD (Q9NZQ3-3) (Met1-Thr244) was expressed with the GST tag at the N-terminus.
表达宿主：E. coli
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：Please contact us for more information.
预测N端：Met
蛋白分子量：The recombinant human NCKIPSD consists of 478 amino acids and predicts a molecular mass of 53.2 KDa. It migrates as an approximately 53 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号：Q9NZQ3-3
蛋白氨基酸序列：Met1-Thr244
蛋白标签：N-GST
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验GST fusion protein purification--GST融合蛋白纯化
GST fusion protein purification GST融合蛋白纯化John Mundy,Institute of Molecular Biology,Copenhagen,Denmark1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze
Purification of GST fusion proteins in E.coli GST融合蛋白纯化--纯化小量蛋白Sugden lab,McArdle Laboratory for Cancer Research,University of Wisconsin-Madison Medical SchoolSmall scale fusion protein preparationGrow 5ml culture o/n in TB with amp.Add o/n culture
原理 GST 纯化系统是利用GST （glutathione-S-transferase ）融合蛋白与固定的谷胱甘肽（GSH）通过硫键共价亲和，通过GSH交换洗脱的原理来进行纯化 。1ml树脂大约可结合5-8 mg融合蛋白，并可反复使用数次。试剂u IPTG（异丙基硫代-β-D-半乳糖苷） 2g IPTG 溶解入10ml 水，过滤除菌，分装，-20℃保存。u Lysis buffer （50ml）1)2.5ml 1 M Tris，pH8.0 2)0.1ml 0.5ml EDTA 3)0.292
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