重组大鼠 PD-L1 / B7-H1 / CD274 蛋白 (ECD, His标签)(产品说明)
蛋白名称:Rat PD-L1 / B7-H1 / CD274 Protein (ECD, His Tag)
蛋白构建:A DNA sequence encoding the rat CD274 (NP_001178883.1) (Met1-Thr238) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized Rat PD-L1 His (Cat:80450-R08H) at 2 μg/ml (100 μl/well) can bind Rat PD-1 hFc (Cat:80448-R02H), the EC50 of Rat PD-1 hFc is 15-50ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 18
蛋白分子量:The recombinant rat CD274 comprises 232 amino acids and predicts a molecular mass of 26.5 kDa. The apparent molecular mass of the recombinant protein is approximately 50.7 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001178883.1
蛋白氨基酸序列:Met1-Thr238
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.