重组大鼠 PD-L1 / B7-H1 / CD274 蛋白 (Fc标签)(产品说明)
蛋白名称:Rat PD-L1 / B7-H1 / CD274 Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the rat CD274 (NP_001178883.1)(Met1-Thr238) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA.Immobilized rat CD274-Fc at 10 μg/ml (100 μl/well) can bind biotinylated rat PDCD1-Fc (Cat:80448-R02H), The EC50 of biotinylated rat PDCD1-Fc (Cat:80448-R02H) is 0.14-0.34 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 18
蛋白分子量:The recombinant rat CD274/Fc is a disulfide-linked homodimer. The reduced monomer comprises 459 amino acids and has a predicted molecular mass of 51.8 kDa. The apparent molecular mass of the protein is approximately 73.2 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001178883.1
蛋白氨基酸序列:Met1-Thr238
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.