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- 规格:
100 µg
蛋白名称:Neuregulin-1蛋白, Neuregulin-1 protein
蛋白构建:A DNA sequence encoding the canine NRG1 (XP_858147.1) extracellular domain (Ser19-Lys240) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is typically 0.2-2 µg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ser 19
蛋白分子量:The recombinant canine NRG1 comprises 233 amino acids and has a predicted molecular mass of 25.5 kDa. The apparent molecular mass of the protein is approximately 39-46 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:XP_858147.1
蛋白氨基酸序列:Ser19-Lys240
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Modeling Schizophrenia in Neuregulin 1 and ErbB4 Mutant Mice
. However, genetic studies have recently identified many susceptibility genes of this disorder including neuregulin 1 (NRG1 ) and its receptor ErbB4 . Arguably, relevant mutant mice have provided a unique opportunity to model “schizophrenia” in animals
可以作用于几个NRG。 bigqi 在pubmed的mesh中也找不到NRG β1. 在文章里找到这样一句话: ...soluble ErbB ligands from the β-exon-containing type I neuregulin-1a protein (also called heregulin, glial growth factor, or ARIA), which we call NRG β1. 名字是他起的?按字面
质,小分子或是金属。当融合有亲和标签的蛋白通过标签-配体作用结合再层析基质上时,通过洗杂步骤,即可去除掉其他的细胞成分。 为了洗脱所需要的蛋白质,通过改变缓冲液的条件,如pH,或通过竞争亲和标签和配体连接的方法来进行。 但怎样的纯化是较为成功的呢?仅达到所需的质量量级,如毫克级(或克级)即可满足吗? 这些并不简单。质在蛋白纯化中同样尤为重要,比如,就蛋白的生物活性和纯度而言,通常会有所要求。 以下我们来讨论并且比较2种技术: His-tag系统和Strep-tag®系统,都使用了亲和层析法来纯化
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