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文献和实验which are bound to the ternary complexes. This is followed by a set of biochemical reactions and structural changes leading to Chl synthesis that can be monitored with fluorescence techniques. This chapter describes the application of low-temperature fluorescence
antibody with DIG (ChromaLink™ Digoxigenin). For IHC staining, cells or tissue sections are incubated first with unconjugated primary antibody against the first target protein followed by detection with antiprimary secondary antibody conjugated
Fluorochrome Absorption and Emission Spectra
laser which can be conveniently tuned to emit light in the 550-650 nm range. (In a dye laser, the lasing medium is a solution of fluorescent dye excited by a pump laser, usually an ion laser.) Texas Red ™ , available conjugated to avidin, can be excited
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