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文献和实验High Throughput Methods in Proteomics
David Wishart University of Alberta Edmonton, AB david.wishart@ualberta.ca Proteomics Proteomics employs an incredibly diverse range of technologies including:
Fluorochrome Absorption and Emission Spectra
., RED613™ , EDC, PerCP), BD Cy-Chrome™ is a superior fluorochrome for third color analysis because of its high emission intensity and broad spectrum. As with our R-PE conjugates, an average of one BD Cy-Chrome™ molecule is coupled per antibody or protein
antibody with DIG (ChromaLink™ Digoxigenin). For IHC staining, cells or tissue sections are incubated first with unconjugated primary antibody against the first target protein followed by detection with antiprimary secondary antibody conjugated
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