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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Phospho-Src (Tyr527) Antibody
- 是否单克隆:
多克隆
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文献和实验(4) 而 527 位 Tyr 的磷酸化,当受体无活性时, Src 527-Tyr 自身磷酸化并与 SH2 区结合,抑制 416 位点磷酸化,受体活化后 , 特异位点的 Tyr 磷酸化并与 Src 的 SH2 结合, Src 的 527 从 SH2 区释放出,并去磷酸化,而 Tyr-416 位点失去抑制而磷酸化, Src 激酶活化。 v-src 和 c-src 的 DNA 顺序基本相同,但 v-src 无内含子,它比 c-src 少编码了 7 个氨基酸( C 端),且 C
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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