High Pure RNA Paraffin Kit

High Pure RNA Paraffin Kit
kit of for up to 100 isolations

一般描述

Low to medium throughput RNA isolation from FFPE sample materials.

应用

The High Pure RNA Paraffin Kit isolates total RNA from paraffin embedded, fresh-frozen tissue. The quality of RNA obtained is suitable for relative quantification of mRNA with RT-PCR, especially on the Lightcycler^® Carousel-Based System. Additional applications include:

• RT-PCR
• Differential display RT-PCR
• cDNA synthesis
• Primer extension

特点和优势

The High Pure RNA Paraffin Kit isolates total RNA from formalin-fixed, paraffin-embedded tissue as well as from fresh-frozen tissue-research samples for direct use in RT-PCR.

• Isolate RNA suitable for RT-PCR from archived paraffin tissue (up to 15 years old).
• Obtain a concentrated product that is ready-to-use (no RNA precipitation required).
• Quickly prepare total RNA from tissue sections in approximately 2 hours (after an overnight digestion).
• Obtain highly pure RNA for use in relative mRNA quantification procedures (e.g ., with the Lightcycler^® System).
• Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.

组分

• Tissue Lysis Buffer
• Proteinase K, PCR-grade
• Binding Buffer
• Wash Buffer I
• Wash Buffer II
• DNase I
• DNase Incubation Buffer
• Elution Buffer
• High Pure Filter Tubes
• Collection Tubes