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文献和实验Kingfisher Flex 96 Plant DNA High Pure Protocol
-well microplate 3. (Optional)Incubator equilibrated to 65°C 4. Equipment for disrupting Plant tissue (MM300 Mixer Mill or Geno/Grinder 2000 and Tungsten carbide beads) or Liquid Nitrogen 5. 8-or 12-channel pipette 6. Reagent reservoir
Preparation of Bacteriophage Lysates and Pure DNA
Preparation of pure bacteriophage DNA used to rely on using CsCl gradients to give high purity or methods that yielded DNA that was either of low recovery or subject to significant genomic contamination. Recently though, new methods have come
E.Z.N.A. Cycle-Pure Kit Spin Protocol
. 9. Place HiBind® DNA column into a clean 1.5ml microcentrifuge tube. Add 30-50ul (depending on desired concentration of final product) of Elution Buffer (10mM Tris, pH8.5) or water directly onto the column matrix and centrifuge for 1 min at 13,000
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