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Neobioscience
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文献和实验Chromatin Immunoprecipitation (ChIP)
of the method requires ~2 h, followed by an overnight incubation. 17. Add 50 µL of protein A-agarose/salmon sperm DNA or protein G-agarose/salmon sperm DNA (depending on antibody) beads to the chromatin samples on ice. Rotate for 2 h at 4°C
Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
). Day 2 14. If you are not using rabbit polyclonal antibodies (ie. monoclonal antibodies, ect), add 1 ug of an appropriate secondary antibody (rabbit) and incubate for an additional 1 hour. 15. Add
Methods for use with the mTn-3xHA/GFP-mutagenized library
of the GAL1-10 promoter on galactose. Our strains grow poorly on galactose but give 80 to 100% popouts. The HA triple tag can be detected by mouse monoclonal antibodies 12CA5 (Boehringer) or MMS101R (BAbCo, Richmond, California). These antibody recognise
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