NG, NG-Dimethyl-L-arginine . d

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

.    Rao, F., Pasunooti, S., Ng, Y., Zhuo, W., Lim, L., Liu, A.W., and Liang, Z.–X. 2009. Enzymatic synthesis of c‐di‐GMP using a thermophilic diguanylate cyclase. Anal. Biochem. 389:138‐142

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

if aliquoted. Do not mix before use. Positive control uses 10ng supercoiled plasmid   END LABELLED PROBE PREPARATION 1) Digest plasmid w/ 5''overhang enzyme 2) P/CHCl3 ext 3) EtOH ppt 4) Redissolve to 0.5mg/ml in H2O 5) Start

【共享】慢病毒载体

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