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- Invivogen
- ant-zn-5p
- 美国
- 2026年03月05日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- CAS号：
11006-33-0
- 保质期：
见包装说明
- 英文名：
Zeocin™
- 供应商：
欣博盛
- 库存：
大量
- 保存条件：
Room temperature
- 规格：
5 g (powder)
Zeocin™ (powder)/ant-zn-5p/5g 品牌：Invivogen；产品名称：Zeocin™ (powder)；货号：ant-zn-5p；规格：5g (powder)
Zeocin是Phleomycin D1的商业名称，是从轮枝链霉菌Streptomyces CL990分离得到的一种铜螯合的糖肽抗生素，Zeocin通过嵌入DNA使其断裂而导致细胞死亡。Zeocin在细菌、真核微生物、植物细胞和哺乳动物细胞上均能发挥活性，用于筛选携带Sh ble基因的转染细胞。
Zeocin化学特性
CAS号：11006-33-0
分子式：C55H85O21N20S2Cu - HCl
分子量：1525
推荐使用浓度
大肠杆菌
Zeocin抗性的转化子可以在低盐LB琼脂糖培养基(yeast extract 5 g/l, Tryptone 10 g/l, NaCl 5 g/l, Agar 15 g/l,pH 7.5)，25 ug/ml Zeocin的条件下筛选。invivogen公司的FastMedia™ Zeo培养基可助您得到最佳筛选效果。
哺乳动物细胞
| 细胞系 | 种属 | 组织 | 培养基 | Zeocin(ug/ml) |
| B16 | mouse | melanoma | RPMI | 20-250 |
| CHO | hamster | ovary | DMEM | 100-500 |
| COS | monkey | kidney | DMEM | 100-400 |
| 293 | human | kidney | DMEM | 100-400 |
| Hela | human | uterus | DMEM | 50-100 |
| J558L | mouse | melanoma | RPMI | 400 |
| MCF-7 | human | Breast adenocarcinoma | DMEM | 100-400 |
| MEFs | mouse | embryonic fibroblasts | DMEM | 200-400 |
| THP-1 | human | Leukemic monocytes | RPMI | 200 |
质量控制：
纯度>90%(HPLC)
活性经微生物学实验鉴定
保存
4°C或-20°C保存1年，避免反复冻融。
产品信息:
| 产品名称 | 产品编号 | 规格 | 品牌 | 折扣 |
| Zeocin™ | ant-zn-1 | 1g (10 x 1 ml) | Invivogen | 请询 |
| Zeocin™ | ant-zn-5 | 5g (50 x 1 ml) | Invivogen | 请询 |
| Zeocin™ | ant-zn-5b | 5 g (50 ml bottle) | Invivogen | 请询 |
| Zeocin™ | ant-zn-1p | 1 g (powder) | Invivogen | 请询 |
| Zeocin™ | ant-zn-5p | 5 g (powder) | Invivogen | 请询 |
文献引用
•2011 - PNAS, 108: 16663 - 16668
CD147 subunit of lactate/H+ symporters MCT1 and hypoxia-inducible MCT4 is critical for energetics and growth of glycolytic tumors.
Le Floch R, Chiche J, et al.
•2011 - Infect. Immun., 79: 1418 - 1427
NOD-like receptor activation by outer membrane vesicles from Vibrio cholerae non-O1 non-O139 strains is modulated by the quorum-sensing regulator HapR.
Bielig H, Rompikuntal PK, et al.
•2012 - PNAS, 109: 78 - 83
Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.
Sandstrom AG, Wikmark Y, et al.
Invivogen公司是一家基因治疗相关的分子生物学试剂公司，公司3213年在美国加州生物公司聚集地圣地亚哥成立，初期即以其拳头产品支原体抗生素与同城Invitrogen公司合作进行全球市场推广。其主打产品有：多基因表达质粒载体、DNA疫苗质粒载体、鼠类转基因质粒载体、合成基因质粒载体pMOD、腺病毒载体AdenoVec、基因转染试剂和一些非常具有特色的试剂盒，此外该公司还拥有很大的基因和启动子库，可以接受客户的定制服务，极大地方便了客户。
详情请咨询invivogen全国授权一级代理-欣博盛生物科技
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- 询问日期
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of a sample as negative despite the analyte being present, and hence is to be avoided. CANDOR’s SmartBlock™ is a surface blocker that prevents the described mechanism leading to the covering of epitopes and is hence especially suited for use with small
Isolation of RNA from Difficult Tissues
ToTALLY RNA™ Kit). Note that many of these techniques can be used with other RNA isolation protocols as well. Heart and Skeletal Muscle: Fibrous Tissues For fibrous tissues such as rat and mouse heart and skeletal muscle, the most difficult step
from powder) in Buffer A (dissolve the p-formaldehyde by putting the solution in boiling water bath for few minutes). Use this solution to transfer the embryos to a glass flask, or transfer the embryos with a paintbrush into a vial that already contains
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