PCR PIPET TIPS 0.5-25uL

is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea

  The gene we are interested in looking at is not very abundant. At 100 ng/ul, the CT value is 30.9 In the literature that I have looked at, people seems to using much lower levels of cDNA. I would actually

SQ Blood Mini Protocol for 50ul Clotted Blood

2. Nuclease-free 1.5 ml microcentrifuge tubes 3. Water Bath preset at 37°C 实验步骤   1. Transfer the 50ul blood include any liquid residual into a 1.5 ml centrifuge tube. 2. Add 550ul WTL Buffer and pipet

PCR基础知识[PCR basics]

itself as the predominant template. Cycles are typically repeated 25 to 45 times. Standardisation of the thermocycler's running conditions is essential for the reproducibility of results.PCR procedures: conditions for cycling ! Complete denaturation of the DNA template