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- 文献和实验
- 技术资料
- 英文名：
FILTER TIPS,350UL
- 保存条件：
Invalid
- 供应商：
西宝生物
- 库存：
10 x 100/box
- 规格：
LABWARE
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文献和实验ensures a low background level of nonspecific probe adherence to the membrane and, second, an extended period of filter prehybridization is not required. The inclusion of a large amount of SDS does, however, necessitate that the nucleic acids
binds to a specific DNA sequence, passage through the filter will result in retention of a fraction of the protein-DNA complex by virtue of the protein component of the complex. The amount of DNA retained can be determined by using radioactively labeled
Filter Binding Assay for EBNA-1
, 3ml syringePre-incubate filter with 100ul Binding Buffer at filter binding temperature (4C) until ready to bind DNA/EBNA-1 solution (e.g. conduct in 7th floor cold room).Incubate DNA fragment of interest with DNA with no known binding sites in a total
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