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- 文献和实验
- 技术资料
货号:4389764
产品名称:BTL,SOLiD 1X LOW TE BUFFER
品牌:abi
关键字:abi,Applied Biosystems公司,4389764,BTL,SOLiD 1X LOW TE BUFFER
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文献和实验Preparation of Mycoplasma chromosomal DNA in low melting agarose
. prewarmed). 6. Place on ice for 3-5 minutes and transfer the solid plug into polystyrene tube containing 0.5 ml 2% tween-20 in TE 7. Incubate at 37 C overnight. 8. Dialyse the plug in TE at 4 C for at least four hours. Change buffer every 1 hour. TNE: 10
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
shaker. 4. At 1hr and 50 minutes after the fixing start time: o Spin tubes @ 2K for 5 min, RT. o Decant and blot on paper towel. o Vortex pellet. o Resuspend pellet in about 25ml of 1X Buffer A (TBS). o Repeat steps 1 to 4. o Repeat steps 1 to 3
DNA Recovery With Low Melt Agarose
Recovery of DNA from Low Melting Point Agarose Gels 1.Run digestion products on 0.7% LMP agarose gel in 1X TBE (it's nice to have at least 1ug of the fragment you want). LMP agarose is fragile; pour gel with EtBr 2.Let solidify in cold room
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