BTL,SOLiD 1X LOW SALT BINDING

Salt (KCl) concentration

7, where both primers have a similar melting point with primers for locus 6 (58° C). They are not as well amplified in 1x buffer, but respond well to increase in the salt concentration. In this case, the explanation may be that the entire product 7 has a lower GC

Determination of Sequence Preferences of DNA Binding Proteins Using Pooled Solid-Phase Sequencing of Low Degeneracy Oligonucleotide Mixtures

can be selected by protein binding. Strategies based on this approach, however, typically are quite laborious: They involve cloning of the oligonucleotides after the selection step and sequencing of a great number of individual clones to yield statistically

Solid Phase PCR Sequencing of Biotinylated Products

. Attempts to improve this by the precipitation of M 13 phage with acetic acid and the recovery and subsequent disruption of the phage on glass fiber disks have been described (4 ). The DNA is eluted with a low salt buffer and requires neither phenol