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货号:AM9520
产品名称:Linear Acrylamide 5 x 1 ml
品牌:abi
关键字:abi,Applied Biosystems公司,AM9520,Linear Acrylamide 5 x 1 ml
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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
Preparation of linear polyacrylamide
,and dissolve the pellet in10 mM Tris-HCl (pH8.0),1 mM EDTA to make a final polyacrylamide concentration of 5 mg/ml. 6.The linear polyacrylamide solution can be stored in the refrigerator for several years. 7.Add 10-20 micrograms of the linear
OLIGO PURIFICATION on acrylamide gels
dried-down oligo in 40μl TE. Add 20μl formamide dye. 3. Prepare a 12% polyacrylamide gel (20:1 acrylamide:bis-acrylamide). 6.3ml 20x TBE 32ml 7M urea 38ml 40% acrylamide/7M urea 50ml dH2O 375μl ammonium persulphate 75μl TEMED 4. Cast gel in 25 x
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