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文献和实验Silver Staining Protocol for Acrylamide Gels
) 453 gms 10X TBE 50 ml 40%(19:1 ratio) Acrylamide : bisacrylamide
Preparation of linear polyacrylamide
,and dissolve the pellet in10 mM Tris-HCl (pH8.0),1 mM EDTA to make a final polyacrylamide concentration of 5 mg/ml. 6.The linear polyacrylamide solution can be stored in the refrigerator for several years. 7.Add 10-20 micrograms of the linear
Linear amplification of limiting amounts of RNA for gene expression studies
, and then add 5mg of carrier tRNA. Add 100µl of CHCI3 and centrifuge at 13,000rpm for 10 minutes; Transfer the upper phase into a new 1.5ml tube, and add 700ml of isopropanol; Precipitate the RNA by either putting the tube at -20°C o/n or at -80°
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